A new history of molecular biology re-inscribed. Now the ability of an electron microscope to capture images intact double-stranded structure of DNA (deoxyribonucleic acid). This advanced technique allows researchers to see the interaction of proteins, RNA (ribonucleic acid), with DNA and other biomolecules.
Initially, the structure of DNA was discovered by using the crystallography X-ray. Emitted X-ray scattering to extract DNA atoms crystallized to form a complex pattern of dots on a sheet of film. However, interpreting the images to determine the specific pattern of the crystal structure of DNA requires complex mathematics.
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| DNA's double-helix structure is on display for the first time in this electron microscope photograph of a small bundle of DNA strands. (PIcture from: http://www.livescience.com/) |
"The new image is much clearer because it is a direct illustration of strands of DNA," said Enzo di Fabrizio, researchers at the University of Genoa, Italy, on Thursday last week.
There is a special trick to obtain a complete image of DNA. Di Fabrizio and his team tore double strand of DNA from dilute solution and placed on a silicon board nanoscopik. Board designed waterproof silicone, making the solution easy to evaporate and leave only the DNA strands.
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| A bundle of DNA is supported by two silicon pillars. (PIcture from: http://www.livescience.com/) |
The radiation from the electron microscope and then target DNA strands of nanoscale holes in the base of the board to obtain high-resolution imagery. "The results show the structure of the DNA double strands evident," said Di Fabrizio.
Di Fabrizio said the use of more sensitive detectors that can respond to a low-energy electrons. This allows researchers not only to see double-stranded structure, but also single individual DNA strands. "Samples are well prepared and have improved imaging resolution," he said.
Earlier this year, a team of University College London researchers led by Bart Hoogenboom also found a way to take pictures of individual DNA strands. They use Braille technique utilizing atomic force microscopy. As the Italian team, Hoogenboom and his colleagues are also able to detect circular pattern that separates the twin strands of DNA. *** [NEWSCIENTIST | LIVESCIENCE | MAHARDIKA SATRIA HADI | KORAN TEMPO 4074]
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